Abstract
Electrochemiluminescence (ECL) is nowadays a powerful technique widely used in biosensing and imaging, offering high sensitivity and specificity for detecting and mapping biomolecules. Screen-printed electrodes (SPEs) offer a versatile and cost-effective platform for ECL applications due to their ease of fabrication, disposability, and suitability for large-scale production. This research introduces a novel method for improving the ECL characteristics of screen-printed carbon electrodes (SPCEs) through the application of CO(2) laser treatment following fabrication. Using advanced ECL microscopy, we analyze three distinct carbon paste-based electrodes and show that low-energy laser exposure (ranging from 7 to 12 mJ·cm(-2)) enhances the electrochemical performance of the electrodes. This enhancement results from the selective removal of surface binders and contaminants achieved by the laser treatment. We employed ECL microscopy to characterize the ECL emission using a bead-based system incorporating magnetic microbeads, like those used in commercial platforms. This approach enabled high-resolution spatial mapping of the electrode surface, offering valuable insights into its electrochemical performance. Through quantitative assessment using a photomultiplier tube (PMT), it was observed that GST electrodes could detect biomarkers with high sensitivity, achieving an approximate detection limit (LOD) of 11 antibodies per μm(2). These findings emphasize the potential of laser-modified GST electrodes in enabling highly sensitive electrochemiluminescent immunoassays and various biosensing applications.