Abstract
OBJECTIVE: To examine whether early β-amyloid (Aβ) accumulation and metabolic risk factors are associated with neuroinflammation in elderly individuals without dementia. METHODS: We examined 54 volunteers (mean age 70.0 years, 56% women, 51% APOE ɛ4 carriers) with the translocator protein (TSPO) tracer [(11)C]PBR28 to assess neuroinflammation and with [(11)C] Pittsburgh compound B (PiB) to assess cerebral Aβ accumulation. [(11)C]PBR28 and [(11)C]PiB standardized uptake value ratios (SUVRs) were quantified in 6 regions of interests by using the cerebellar cortex as a pseudo-reference and reference region, respectively. Fasting venous glucose, insulin, and high-sensitivity C-reactive protein (hs-CRP) values were determined. Homeostatic model assessment of insulin resistance (HOMA-IR) was calculated. A subset of individuals (n = 11) underwent CSF sampling, and Aβ(40), Aβ(42), total tau, phospho-tau, soluble TREM2, and YKL-40 levels were measured. RESULTS: Among the whole study group, no significant association was found between [(11)C]PiB and [(11)C]PBR28 SUVR composite scores (slope 0.02, p = 0.30). However, higher [(11)C]PiB binding was associated with higher [(11)C]PBR28 binding among amyloid-negative ([(11)C]PiB composite score ≤1.5) (TSPO genotype-, age- and sex-adjusted slope 0.26, p = 0.008) but not among amyloid-positive (slope -0.004, p = 0.88) participants. Higher CSF soluble TREM2 (r (s) = 0.72, p = 0.01) and YKL-40 (r (s) = 0.63, p = 0.04) concentrations were associated with a higher [(11)C]PBR28 composite score. Higher body mass index, HOMA-IR, and hs-CRP were associated with higher [(11)C]PBR28 binding in brain regions where Aβ accumulation is first detected in Alzheimer disease. CONCLUSIONS: While there was no association between amyloid and neuroinflammation in the overall study group, neuroinflammation was associated with amyloid among the subgroup at early stages of amyloid pathology.