Abstract
PURPOSE: Infection is a common complication following left ventricular assist device (LVAD) implantation. Patients with obesity are particularly at risk due to their high percentage of adipose tissue and the resulting chronic inflammatory state and resulting immunological changes. This study investigated changes of immunological parameters in relation to body mass index (BMI) during the first year after LVAD implantation. METHODS: Blood samples were obtained prior to LVAD implantation and at 3 (1(st) FU), 6 (2(nd) FU) and 12 mo (3(rd) FU) after LVAD implantation. Patients were divided into three groups (normal weight: BMI of 18.5-24.9 kg/m(2); n=12; pre-obesity: 25.0-29.9 kg/m(2); n=15; obesity: ≥ 30.0 kg/m(2); n=17) based on their BMI at the time of LVAD implantation. Flow cytometric analyses for CD4(+) and CD8(+) T cells, regulatory T cells (T(regs)), B cells as well as dendritic cells (DCs) were performed. RESULTS: After LVAD implantation, obese patients (0.51 ± 0.20%) showed a higher proportion of overall DCs than normal-weight (0.28 ± 0.10%) and pre-obese patients (0.32 ± 0.11%, p<0.01) at 3(rd) FU. The proportion of BDCA3(+) myeloid DCs was lower in obese patients (64.3 ± 26.5%) compared to normal-weight patients (82.7 ± 10.0%, p(normal-weight vs. obesity)=0.05) at 2(nd) FU after LVAD implantation. The analysis of BDCA4(+) plasmacytoid DCs revealed a reduced proportion in pre-obese (21.1 ± 9.8%, p(normal-weight vs. pre-obesity)=0.01) and obese patients (23.7 ± 10.6%, p(normal-weight vs. obesity)=0.05) compared to normal-weight patients (33.1 ± 8.2%) in the 1(st) FU. T cell analysis showed that CD4(+) T cells of obese patients (62.4 ± 9.0%) significantly increased in comparison to pre-obese patients (52.7 ± 10.0%, p(pre-obesity vs. obesity)=0.05) and CD8(+) T cells were lower in obese patients (31.8 ± 8.5%) than in normal-weight patients (42.4 ± 14.2%; p(normal-weight vs. obesity)=0.04) at the 3(rd) FU. Furthermore, we observed significantly reduced proportions of T(regs) in pre-obese patients compared to normal-weight and obese patients at 2(nd) FU (p=0.02) and 3(rd) FU (p=0.01) after LVAD implantation. CONCLUSION: This study reported changes of the innate and adaptive immune system of pre-obese and obese compared to normal-weight patients one year after LVAD implantation. DCs and their subsets, CD8(+) T cells and T(regs) were affected immune cell populations that indicate immunological changes which might increase the incidence of postoperative infection.