Protocol for the operation of a breathing and vaping biomimetic robot to delineate real-time inhaled particle profile of electronic cigarettes

We recently developed a robotic human vaping mimetic real-time particle analyzer (HUMITIPAA) to evaluate the impact of change in chemical constituents and breathing profiles of electronic cigarettes (ECs) on potential pulmonary toxicity. Here, we describe the fabrication procedure of EC mouthpiece(s), establishment of sensor saturation curve, and preparation of e-liquid and vaping device(s) for testing. We further detail steps for HUMITIPAA preparation and connection setup, followed by data collection and processing. For complete details on the use and execution of this protocol, please refer to Kaiser et al. (2021).1.