Abstract
The medial entorhinal cortex (MEC) is crucial for context memory, yet its role in context-induced retrieval of morphine withdrawal memory remains to be investigated. Here, we present a protocol to evaluate the importance of projection neurons from the MEC to the basolateral amygdala (BLA) (MEC-BLA neurons) in mice during context-induced retrieval of morphine withdrawal memory using a conditioned place aversion (CPA) model. We describe steps for surgical procedure and behavioral experiments. Then, we detail procedures of immunofluorescence staining and image analysis. For complete details on the use and execution of this protocol, please refer to Fu et al.1.