Abstract
BACKGROUND: This research is aimed to study the resistance and molecular epidemiological characteristics of carbapenem-resistant Klebsiella pneumoniae (CRKP). METHODOLOGY: 38 isolated CRKP strains were collected from clinical specimens. RESULTS: The resistance rates were more than 70.0%. Ampicillin had the highest rates among them (100.0%). 34 strains (89.5%) among the 38 CRKP strains carried bla (KPC-2) gene, and 3 strains (7.9%) carried bla (IMP-4) gene. 36 strains (94.7%) among the 38 CRKP strains carried bla (SHV) gene, 29 strains (76.3%) carried bla (TEM) gene, and 26 strains (68.4%) carried bla (CT-M) gene. 7 strains (18.4%) among the 38 CRKP strains carried bla (DHA-1) gene. 15 strains (39.5%) in 38 CRKP strains lost two fenestra proteins, ompK35 and ompK36, and the rest 23 strains carried ompK36 genes. 38 CRKP strains were divided into five kinds of ST types, with ST11 type as the most (86.8%, 33/38). The rest of the ST types included 2 strains of ST23 (5.3%, 2/38), one strain of ST15, ST1373 and ST1415 (2.6%, 1/38). CONCLUSIONS: CRKP resistance is severe, and the mechanism of drug resistance has become increasingly complex. Various ST types and resistance genes are related to CRKP. The clinical prevention and control work is imminent.