Enzyme immunoassays for water-soluble steroid metabolites in the urine and feces of Japanese macaques (Macaca fuscata) using a simple elution method

采用简单洗脱法对日本猕猴(Macaca fuscata)尿液和粪便中的水溶性类固醇代谢物进行酶联免疫测定

阅读:14
作者:

Abstract

Keiko SHIMIZU and Keiko MOURI Vol. 80, No. 7 (2018), p. 1139, the number of the Plate preparation section should have been as follows:I ErrorI Plate preparation. Anti-rabbit IgG (H+L) goat serum (Rockland Immunochemicals Inc., Limerick, PA, U.S.A.) was diluted to 0.15 μg/ml in pH 9.6, 0.05 M carbonate buffer, and 50 μl was added to microtiter plates (Maxisorp flat-bottom, Thermo Fisher Scientific, Waltham, MA, U.S.A.) for the E1C, PdG and E3G assays.II CorrectionI Plate preparation. Anti-rabbit IgG (H+L) goat serum (Rockland Immunochemicals Inc., Limerick, PA, U.S.A.) was diluted to 15 μg/ml in pH 9.6, 0.05 M carbonate buffer, and 50 μl was added to microtiter plates (Maxisorp flat-bottom, Thermo Fisher Scientific, Waltham, MA, U.S.A.) for the E1C, PdG and E3G assays.

特别声明

1、本页面内容包含部分的内容是基于公开信息的合理引用;引用内容仅为补充信息,不代表本站立场。

2、若认为本页面引用内容涉及侵权,请及时与本站联系,我们将第一时间处理。

3、其他媒体/个人如需使用本页面原创内容,需注明“来源:[生知库]”并获得授权;使用引用内容的,需自行联系原作者获得许可。

4、投稿及合作请联系:info@biocloudy.com。