Abstract
Cynanchum auriculatum is a Chinese herbal medicine species in the family Apocynaceae. The mitochondrial genome of C. auriculatum has heteroplasmy and consists of two chromosomes (chromosomes I and II), the lengths of which are 614,836 and 426,495 nucleotides. The multipartite mitochondrial genome encodes 57 genes, including 37 protein-coding genes, 17 transfer RNA genes, and three ribosomal RNA genes. Including 44 overlapping genes, we identified 57 genes on chromosome I and 44 genes on chromosome II. A phylogenetic tree revealed that C. auriculatum is most closely related to Asclepias syriaca.