Abstract
We present a protocol to generate a liver tumor murine model utilizing orthotopic ultrasound-guided intrahepatic injections of tumor cells and a partial hepatectomy. We describe cell and animal preparation and ultrasound-guided intrahepatic injections. We then depict how to conduct a partial hepatectomy addressing solutions to potential problems. This approach introduces a rapid and reproducible orthotopic implantation model of liver tumor model in a minimally invasive manner, allowing the surgical procedure to be optimized with minimal intra-abdominal injury. For complete details on the use and execution of this protocol, please refer to Brown et al.(1).