Abstract
In this protocol, we detail steps for constructing a high-throughput automated platform for thin layer chromatography (TLC) analysis. We describe robotics and computer vision techniques that can handle 32 compounds under three different elution solvents in about 50 min. The established automated platform can obtain statistically standardized retardation factor (R(f)) values and enhance reproducibility while reducing labor and time costs. For complete details on the use and execution of this protocol, please refer to Xu et al. (2022).(1).