Abstract
This study aimed to explore a valid test protocol for measuring VO(2max) in healthy untrained male Wistar rats of different ages and quantifying the exercise intensity (%VO(2max)) of running under different treadmill grades and speeds. The test protocols and %VO(2max) will provide a reference for the design of exercise intensity. We tested male Wistar rats aged 4 weeks, 10 weeks, 10 months and 16 months old with three test protocols (Procedure 1 [P1], 2 [P2] and 3 [P3]) for each age group to quantify VO(2max). We analysed VO(2max), respiratory exchange ratio and test duration to determine an optimal test protocol of VO(2max) for different age groups. We used the optimal test protocol to explore the changes in age-related VO(2max). Finally, %VO(2max) of running under different treadmill speeds and grades was quantified. VO(2max) of Wistar rats decreased significantly after the age of 4 weeks (p < 0.05). The optimum VO(2max) can be induced by personalised protocols for different ages. In 4-week-old Wistar rats, the highest VO(2max) values were attained by P1 (104.4 ± 6.9 mL · kg(-1) · min(-1), p = 0.032). The highest VO(2max) value (84.7 ± 3.7 mL · kg(-1) · min(-1), p = 0.037) of 8-week-old Wistar rats was attained in P2. In 10-month-old Wistar rats, the highest VO(2max) value was obtained in P3 (63.3 ± 1.7 mL · kg(-1) · min(-1)). This work could be used as a reference for assessing aerobic capacity in studies on exercise intervention with untrained male Wistar rats. However, the %VO(2max) measurements at various treadmill speeds and grades only apply to untrained male Wistar rats.