Abstract
OBJECTIVES: The best indicator of vitamin D level is the determination of serum 25-hydroxyvitamin D (25(OH)D). Due to the lack of standardization of the available methods, there are problems of precision and reproducibility in its measurement. The objective of this study was to compare the results of 25(OH)D concentration determined by two different immunoassay methods. DESIGN: and Methods: 25(OH)D was analyzed in 184 serum samples in an IDS-iSYS Multi-Discipline Automated System Analyzer (Vitro) and in an Alinity i automated Analyzer (Abbott). Then, results were compared. Three groups were considered: group of total patients, group with vitamin D supplements and group without treatment. RESULTS: Of the total patients, 52.7% received vitamin D supplements. The correlation coefficient of agreement for general group, supplementation group and group without supplementation was 0.92, 0.91 and 0.89, respectively. In all of them, a kappa index>0.75 was obtained. CONCLUSIONS: The assays evaluated are not comparable to each other. Despite this, they show an excellent concordance in the evaluation of the vitamin D status.