Immunohistochemical localization of inhibin/activin subunits in adult Asian elephant (Elephas maximus) testes

成年亚洲象(Elephas maximus)睾丸中抑制素/活化素亚基的免疫组织化学定位

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作者:Sirinart Chaichanathong, Kasuyoshi Taya, Gen Watanabe, Kentaro Nagaoka, Worawidh Wajjwalku, Apichaya Sudsukh, Nikorn Thongtip

Abstract

Immunolocalization of inhibin-α and inhibin/activin βA and βB subunits in the testes of Asian elephant was determined. Testicular sections were immunostained with polyclonal antisera against inhibin subunit-α and inhibin/activin βA and βB using the avidin-biotin-peroxidase complex method. Positive immunostaining against inhibin-α subunit was strongly present in Sertoli cells, and positive immunostaining for the inhibin/activin βA and βB subunits was observed in both Sertoli and Leydig cells. These results indicated that while Sertoli cells are the predominant source of inhibin and activin secretions in the testes of adult male Asian elephant, Leydig cells are a source of activin but not inhibin.

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