Molecular characterization of bovine foamy virus and its association with bovine leukemia virus infection in Vietnamese cattle

牛泡沫病毒的分子特征及其与越南牛牛白血病病毒感染的关联

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作者:Dung Thi LE, Son Vu Nguyen, Mari Okamoto, Nanako Yamashita-Kawanishi, Tung Duy Dao, Vuong Nghia Bui, Haruko Ogawa, Kunitoshi Imai, Takeshi Haga

Abstract

The detection of bovine foamy virus (BFV) in Vietnamese cattle was performed using conventional PCR targeting pol and gag genes. Out of 243 tested samples, ten (4.1%) and eight (3.3%) samples were positive for BFV gag and pol DNA, respectively. The prevalence of bovine leukemia virus (BLV) estimated by detection of proviral DNA using nested PCR targeting env gene was 26.7% (65/243). The results of nucleotide sequence alignment and the phylogenetic analysis suggested that Vietnamese BFV strains showed high homology to isolates belonging to either European or non-European clades. There was no significant correlation between BLV and BFV. This study provides information regarding BFV infection and confirms the existence of two BFV clades among Vietnamese cattle for the first time.

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