Abstract
Understanding gastric physiology in rodents is critical for advancing preclinical neurogastroenterology research. However, existing techniques are often invasive, terminal, or limited in resolution. This study aims to develop a non-invasive, standardized MRI protocol capable of capturing whole-stomach dynamics in anesthetized rats with high spatiotemporal resolution. Experiments were performed in a 7-T MRI system. Gadolinium-doped test meals were prepared to enhance intraluminal contrast in T(1)-weighted MRI. Based on a modified multi-slice gradient-echo sequence, our protocol integrates respiratory gating to minimize motion artifacts, spatial saturation to improve intraluminal contrast, and slice grouping to optimize the trade-offs between signal-to-noise ratio and motion sensitivity. Image acquisition was accelerated using a time-interleaved k-space undersampling scheme, with missing data reconstructed through k-t interpolation. Image quality and gastric motility were quantitatively assessed. The protocol enabled successful imaging of the stomach and visualization of its quasi-periodic dynamics in anesthetized rats. The gadolinium-doped meal produced relatively homogeneous intraluminal contrast, allowing clear delineation of gastric anatomy, volume, and motility. The retrospectively reconstructed image exhibited high image quality and yielded reliable estimates of antral contractions, confirming the effectiveness and robustness of the k-t interpolation method. Estimated antral contraction amplitude and velocity showed minimal deviations from the reference values, whereas contraction frequency estimation remained highly consistent and accurate. Using the accelerated imaging protocol, we imaged the entire stomach and major intestinal regions, acquiring 24 slices with an effective temporal resolution of < 3 s and capturing antral contraction at ~5 cycles per minute. We established an accessible and standardized imaging protocol that encompasses contrast meal preparation, animal handling and training, and a contrast-enhanced dynamic GI MRI acquisition and reconstruction framework. This protocol provides a comprehensive, robust, non-invasive tool for studying gastric motility and dysmotility in rodents, offering strong potential to advance preclinical gastrointestinal motility research.