Abstract
The Schizosaccharomyces pombe septation initiation network (SIN) is required for cytokinesis and septation. The SIN includes a protein kinase cascade that is assembled at spindle pole bodies (SPBs) in a cell cycle specific manner on a scaffold consisting of Cdc11 , related to human centriolin, and the a-helical protein Sid4 . Here, we characterized temperature-sensitive cdc11 and sid4 mutants isolated in the 1990s. We determined the mutations within each allele, examined their phenotypes, and analyzed their growth compared with previously characterized mutant alleles. The new mutants described here expand the toolkit for studying how the SIN assembles at SPBs.