Abstract
In recent years, there has been increased attention to exploring non-mammalian model organisms to study the antioxidant properties of bioactive compounds. These models include both unicellular organisms, such as Escherichia coli and Saccharomyces cerevisiae, and multicellular organisms, such as Caenorhabditis elegans, Drosophila melanogaster, and Danio rerio. In particular, multicellular models have emerged as promising systems due to their ease of establishing systems and maintenance, short duration of experiments, ease of genetic manipulation and genome-wide screening, availability as off-the-shelf models, safety, and cost-effectiveness. Notably, these organisms share a high degree of gene homology with humans, ranging from 65% to 84%, which positions them as powerful platforms for investigating human disease mechanisms. These advantages make them attractive candidates for investigating the potential health benefits of various bioactive compounds before resorting to mammalian models. This review delves into the rationale for utilizing these emerging non-mammalian model organisms during preliminary stages of research, emphasizing their distinct advantages over traditional mammalian models. It also highlights their significant contributions to advancing our understanding of the antioxidant mechanisms of bioactive compounds, shedding light on their potential therapeutic implications for human health. By leveraging these models, researchers can efficiently screen and validate bioactive compounds, laying a robust foundation for subsequent translational studies in mammalian systems.