Conclusion
The synergistic effect of TGF-β3 and hydrodynamic shear stress conditions had a positive impact on increasing wound healing and improving the rate of wound closure.
Methods
In the current study, the impact of TGF-β3 in bone cells' wound healing was examined in vitro. Furthermore, the activities and characteristics of TGF-β3, as well as those of related growth factors throughout this wound healing process, were studied under hydrodynamic shear stress conditions as well as static conditions of cultured bone cells.
Results
We demonstrated that a positive outcome of TGF-β3 treatment was found after 24 h under a static condition, while TGF-β3 treatment was found to be effective under a dynamic condition for wound closure. In the case of the dynamic condition, a full wound closure was obtained after 18 h in both the control and TGF-β3 treatment, while in the case of static conditions, wounds were found to remain open, even after 24 h, for both the control and TGF-β3 treatment. Additionally, in the static condition, the wound closure rate with TGF-β3 treatment was found to be quicker than that of the control flask, which implies that wound healing can be postponed in the static condition. In the dynamic condition, the wound healing process became more rapid in a cultured cell environment.