Microsatellite primer development for the invasive perennial herb Gypsophila paniculata (Caryophyllaceae)

入侵多年生草本植物满天星(石竹科)的微卫星引物开发

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作者:Hailee B Leimbach-Maus, Syndell R Parks, Charlyn G Partridge

Conclusions

These markers will be useful in characterizing the genetic structure of invasive populations throughout North America to aid targeted management efforts, and in native Eurasian populations to better understand invasion history. Five of these developed primers also amplified in G. elegans.

Results

We identified 16 polymorphic nuclear microsatellite loci for G. paniculata out of 73 loci that successfully amplified from a primer library created using Illumina sequencing technology. Microsatellite primers were developed to amplify di-, tri-, and tetranucleotide repeats and tested in three invasive populations in Michigan. Conclusions: These markers will be useful in characterizing the genetic structure of invasive populations throughout North America to aid targeted management efforts, and in native Eurasian populations to better understand invasion history. Five of these developed primers also amplified in G. elegans.

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