Abstract
Long-horned beetles are among the major insect pests that can cause significant economic and ecological damage globally. The control of long-horned beetles is crucial to sustain the forest ecosystem. Dastarcus helophoroides, an economically important ectoparasitoid of long-horned beetles, is widely utilized in biological control strategies. However, the number of larval instars in D. helophoroides remains underexplored. Larval instar determination is crucial for constructing growth prediction models and ecological life tables for insect populations. In this study, we analyzed the frequency distribution of head capsule widths utilizing a visual approach, followed by a non-linear least squares (NLLS) estimation, and found that D. helophoroides undergo four larval instars before entering the pupal stage. The theoretical and observed data for each larval instar yielded identical mean Brooks-Dyar's ratios (1.80). Re-correlation of the number of instars with their respective mean head capsule widths using linear regression (R²) verified that no larval instar was missed. The Crosby's growth ratio (1% and 2%) indicates a very low likelihood of misclassifying an instar into an adjacent one. Given that the accurate determination of larval instars is crucial for developing effective control programs and predicting future population levels, our findings provide valuable insights for implementing biological control strategies against long-horned beetles.