Label-free plasmonic-based biosensing using a gold nanohole array chip coated with a wafer-scale deposited WS(2) monolayer

利用涂覆有晶圆级沉积WS(2)单层的金纳米孔阵列芯片进行无标记等离子体生物传感

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Abstract

This paper reports the fabrication, testing and obtained performance of a plasmonic sensor employing a gold (Au) nanohole array chip coated with tungsten disulphide (WS(2)), which is then functionalized for the detection of protein-protein interactions. A key novelty is that the WS(2) was deposited as a monoatomic layer using a wafer-scale synthesis method that successfully provided a film of both high quality and uniform thickness. The deposited WS(2) film was transferred onto a Au nanohole array chip using a novel method and was subsequently functionalized with biotin. The final sensor was tested and it demonstrated efficient real-time and label-free plasmonic detection of biotin-streptavidin coupling. Specifically, compared to a standard (i.e. uncoated) Au nanohole-based sensor, our WS(2)-coated Au nanohole array boosted the spectral shift of the resonance wavelength by ∼190%, resulting in a 7.64-fold improvement of the limit of detection (LOD).

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