Abstract
In this study, a novel method for obtaining high-quality krill oil from wet Antarctic krill by using subcritical dimethyl ether (SDE) was proposed. A response surface design was used to obtain the best SDE extraction parameters. The optimum extraction efficiency of 93.77 ± 0.92% was obtained at a stirring speed of 1030 rpm, temperature of 47 °C and dynamic extraction time of 90 min. Compared with n-hexane, ethanol, supercritical CO(2) and subcritical n-butane extraction, the krill oil extracted by SDE exhibited low peroxide values (1.46 ± 0.26 mmol kg(-1)), high astaxanthin (218.06 ± 4.74 mg kg(-1)), phosphatidylcholine (PC) (33.95 ± 0.65%), and phosphatidylethanolamine (PE) (11.67 ± 0.23%) content. Moreover, krill oil extracted by SDE has high levels of EPA (16.38 ± 0.05%) and DHA (7.91 ± 0.07%). SDE extraction proved to be an efficient and safe method for extraction of quality krill oil from wet Antarctic krill, and it could be a promising method for oil extraction in wet food in future.