Development and multiplexed amplification of SSR markers for Thuja occidentalis (Cupressaceae) using shotgun pyrosequencing

使用散弹枪焦磷酸测序技术开发和多重扩增北美柏科(Thuja occidentalis)的 SSR 标记

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作者:Huaitong Xu, Francine Tremblay, Yves Bergeron

Conclusions

Four sets of multiplex microsatellite loci can be used for future genetic studies, which includes investigating genetic diversity and structure, and fragmentation and regeneration studies.

Results

Sixteen loci were tested on a panel of 24 individuals from different populations. The number of observed alleles ranged from four to 22. Four sets of multiplex PCR for the 16 loci were then carried out on 60 individuals of two populations from islands of FERLD Duparquet Forest, Canada. Mean number of alleles, observed heterozygosity, and expected heterozygosity were respectively 5.75, 0.594, and 0.574 for Island 58, and 5.50, 0.704, and 0.624 for Island 134. • Conclusions: Four sets of multiplex microsatellite loci can be used for future genetic studies, which includes investigating genetic diversity and structure, and fragmentation and regeneration studies.

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