Development and characterization of microsatellite markers for the endangered Amazonian tree Aniba rosaeodora (Lauraceae)

濒危亚马逊树种Aniba rosaeodora(樟科)的微卫星标记的开发和表征

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作者:Rafael C Angrizani, Luis A S Contim, Maristerra R Lemes

Conclusions

The polymorphic microsatellite loci developed for A. rosaeodora showed highly informative content and can be used as a powerful tool in genetic diversity and population structure, gene flow, and mating system studies for conservation purposes.

Results

We used an enriched genomic library method to isolate and characterize 11 nuclear microsatellite loci for A. rosaeodora, which exhibited an average of 9.6 and 8.7 alleles per locus in two populations from central Amazonia. Mean observed and expected heterozygosities over the 11 loci were 0.604 and 0.687, and 0.807 and 0.828, respectively, in the two populations. • Conclusions: The polymorphic microsatellite loci developed for A. rosaeodora showed highly informative content and can be used as a powerful tool in genetic diversity and population structure, gene flow, and mating system studies for conservation purposes.

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