Abstract
Vibrio cholerae is a human pathogen that thrives in estuarine environments. Within the environment and human host, V. cholerae uses the type VI secretion system (T6SS) to inject toxic effectors into neighboring microbes and to establish its replicative niche. V. cholerae strains encode a wide variety of horizontally shared effectors, but pandemic isolates encode an identical set of distinct effectors. Effector set retention in pandemic strains despite mobility between disparate strains suggests that horizontal acquisition of these effectors was crucial for evolving pandemic V. cholerae We attempted to locate the donor of the pandemic effectors to V. cholerae To this end, we identified potential gene transfer events of the pandemic-associated T6SS clusters between a fish pathogen, Vibrio anguillarum, and V. cholerae We supported the likelihood of interaction between these species by demonstrating that homologous effector-immunity pairs from V. cholerae and V. anguillarum can cross-neutralize one another. Thus, V. anguillarum constitutes an environmental reservoir of pandemic-associated V. cholerae T6SS effectors that may have initially facilitated competition between pre-pandemic V. cholerae and V. anguillarum for an environmental niche.