Abstract
Food safety has emerged as a paramount concern in global health, prompting innovative approaches to ensure the safety of people's sustenance. In this study, a novel strategy was devised to fabricate Fe(3)O(4)-ZnO-MnO(2) hybrid nanobiocatalysts, which exhibited remarkable enzymatic activity surpassing that of Horseradish peroxidase (HRP) catalysis. It demonstrated exceptional proficiency in decomposing 3,3',5,5'-tetramethylbenzidine (TMB) without the need for harsh reaction conditions or the aid of H(2)O(2). We established colorimetric detection systems based on Fe(3)O(4)-ZnO-MnO(2)-TMB both for nitrite (NO(2) (-)) and Listeria monocytogenes (LM) in food. Impressively, the detection limit of nitrite reached an astonishingly low level of 0.022 mg L(-1), and the detection limit for LM was determined to be 3.5 cfu mL(-1). These compelling results unequivocally validate the potential of these hybrid nanobiocatalysts to fortify food safety measures. Moreover, they serve as a valuable reference for the colorimetric detection of diverse analytes and the simultaneous detection of multiple targets.