Geneticsbiologiesingle cell and expression analysis for erectile dysfunction and cervical cancer targets

遗传生物学:单细胞和表达分析在勃起功能障碍和宫颈癌靶点中的应用

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Abstract

BACKGROUND: Sexual dysfunction and cervical cancer are genetically and molecularly two complex health problems. Here, we integrate genetic inference and single-cell expression analysis to identify potential genetic targets for sexual dysfunction and cervical cancer, and assess causality of these targets utilizing Mendelian randomization approaches. METHODS: We performed a genome-wide association study (GWAS) to identify genetic variants associated with sexual dysfunction and cervical cancer. Next, we examined the cellular landscape of these variation regions based on scRNAseq data. RESULTS: The study identified several genetic variants that are correlated with sexual dysfunction and cervical cancer, respectively, and these differentially expressed in reproductive and cervical cells. Two-Gene Combination Panel Increased expression of the WISP1 gene was detected in cervical cancer tissues. Twas most highly expressed in T cells, and least well- when cells were proliferating. CONCLUSIONS: The study integrates genetics with single-cell expression to nominate genetic targets for sexual dysfunction and cervical cancer and establishes causal support from Mendelian randomization approach.

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