Curcumin-copper complex nanoparticles as antioxidant nanozymes for acute kidney injury alleviation

姜黄素-铜复合物纳米颗粒作为抗氧化纳米酶用于缓解急性肾损伤

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Abstract

Acute kidney injury (AKI) is a heterogeneous disorder frequently occurring in hospitalized patients with multiple comorbidities. Chemotherapy-associated AKI (e.g., cisplatin-induced AKI, CP-AKI) and rhabdomyolysis-induced AKI (RM-AKI) are initiated by the excessive accumulation of reactive oxygen species (ROS). Herein, metal phenolic networks (MPNs) composed of copper (II) (Cu(2+)), a typical cofactor in the native superoxide dismutase (SOD), and a well-studied natural antioxidant curcumin (Cur) (denoted as Cur-Cu) were fabricated to integrate the ROS-scavenging properties of metal ions and polyphenols. The results indicate that Cur-Cu nanoparticles (NPs) possessed robust antioxidative enzyme-like activities. Meanwhile, Cur-Cu NPs with polyethylene glycol (PEG) covalent modification (Cur-Cu@PEG) abolished the ROS-triggered oxidative damage of HK-2 cells. Moreover, Cur-Cu@PEG displayed acceptable biocompatibility in vivo. Furthermore, Cur-Cu@PEG alleviated CP-AKI and RM-AKI in mice with kidney-targeted delivery. Mechanistically, Cur-Cu@PEG effectively lessened the production of ROS, thereby repressing caspase-3-dependent apoptotic/pyroptotic cell death in the kidneys of AKI mice. Altogether, these results offer a viable approach for synthesizing antioxidant metal phenolic networks mimics to ameliorate ROS-related diseases.

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