Abstract
Glutathione functionalized magnetic 3D covalent organic frameworks combined with molecularly imprinted polymer (magnetic 3D COF-GSH MIPs) were developed for the selective recognition and separation of bovine serum albumin (BSA). Ultrasonication was used to prepare magnetic 3D COFs with high porosity (~1 nm) and a large surface area (373 m(2) g(-1)). The magnetic 3D COF-GSH MIP nanoparticles had an imprinting factor of 4.79, absorption capacity of 429 mg g(-1), magnetic susceptibility of 32 emu g(-)(1), and five adsorption-desorption cycles of stability. The proposed method has the advantages of a shorter equilibrium absorption time (1.5 h), higher magnetic susceptibility (32 emu g(-1)), and larger imprinting factor (4.79) compared with those reported from other studies. The magnetic 3D COF-GSH MIPs used with BSA had selectivity factors of 3.68, 2.76, and 3.30 for lysozyme, ovalbumin, and cytochrome C, respectively. The successful recognition and separation of BSA in a real sample analysis verified the capability of the magnetic 3D COF-GSH MIP nanoparticles.