The use of horseradish peroxidase-lectin conjugates to monitor the purity of plasma-membrane preparations obtained from isolated cells

利用辣根过氧化物酶-凝集素偶联物监测从分离细胞中获得的质膜制剂的纯度

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Abstract

The plasma membranes of viable murine EL4 tumour cells were labelled with horseradish peroxidase-conjugated wheat-germ agglutinin. After disruption of the labelled intact cells, plasma-membrane purification could be monitored by ultrastructural examination of the various fractions for positive reaction product on the membrane vesicles.

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