Optimization of Novel Human Acellular Dermal Dressing Sterilization for Routine Use in Clinical Practice

优化新型人体脱细胞真皮敷料在临床实践中的常规灭菌方法

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作者:Hanna Lewandowska, Andrzej Eljaszewicz, Izabela Poplawska, Marlena Tynecka, Alicja Walewska, Kamil Grubczak, Jordan Holl, Hady Razak Hady, Slawomir Lech Czaban, Joanna Reszec, Grażyna Przybytniak, Wojciech Głuszewski, Jarosław Sadło, Małgorzata Dąbrowska-Gralak, Cezary Kowalewski, Piotr Fiedor, Toma

Methods

immunohistology, immunoblotting, and electron paramagnetic resonance (EPR) spectroscopy. It was shown that radiation sterilization did not change the architecture and three-dimensional structure of hADM; however, it significantly influenced the degradation of collagen fibers and induced the production of free radicals in a dose-dependent manner. More importantly, the observed effects did not disrupt the therapeutic potential of the new transplants. Therefore, radiation sterilization at a dose of 35kGy can ensure high sterility of the dressing while maintaining its therapeutic potential.

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