Abstract
The complete mitochondrial genome of Epinephelus chlorostigma has been determined. It contains a typically conserved structure including 13 protein-coding genes, 22 tRNA genes, two rRNA genes and one control region, and the whole sequence was 16,894 bp in length. The overall base composition is A 28.70%, C 27.99%, G 16.28%, T 27.03%. Except ND6 and eight tRNA genes, all other mitochondrial genes are encoded on the heavy strand. Phylogenetic tree was constructed based on 12 protein-coding genes sequences of 18 Epinephelus species, four species belong to Cephalopholis as outgroup, the result showed that E. chlorostigma is most closely related to Epinephelus areolatus. We sequenced the complete mitochondrial genome of E. chlorostigma to enrich the resource of molecular markers for examination of phylogenetic relationships in Epinephelus.