Structural Analysis of a Trimer of β(2)-Microgloblin Fragment by Molecular Dynamics Simulations

利用分子动力学模拟对β(2)-微球蛋白片段三聚体进行结构分析

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Abstract

A peptide β(2)-m(21-31), which is a fragment from residue 21 to residue 31 of β(2)-microgloblin, is experimentally known to self-assemble and form amyloid fibrils. In order to understand the mechanism of amyloid fibril formations, we applied the replica-exchange molecular dynamics method to the system consisting of three fragments of β(2)-m(21-31). From the analyses on the temperature dependence, we found that there is a clear phase transition temperature in which the peptides aggregate with each other. Moreover, we found by the free energy analyses that there are two major stable states: One of them is like amyloid fibrils and the other is amorphous aggregates.

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