Cholesterol crystals induce inflammatory cytokines expression in a human retinal pigment epithelium cell line by activating the NF-κB pathway

胆固醇晶体通过激活 NF-κB 通路诱导人类视网膜色素上皮细胞系中炎症细胞因子的表达

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作者：Yijun Hu, Haijiang Lin, Bernard Dib, Alp Atik, Peggy Bouzika, Christopher Lin, Yueran Yan, Shibo Tang, Joan W Miller, Demetrios G Vavvas

期刊：

Discovery Medicine

影响因子：

2.000

时间：

2014

起止号：

2014 Jul-Aug;18(97):7-14.

doi：

种属：

Human

研究方向：

信号转导

细胞类型：

上皮细胞

信号通路：

NF-κB

Conclusion

Cholesterol crystals can induce inflammatory cytokine expression in ARPE-19 cells by activating the NF-κB pathway.

Methods

APRE-19 cells were cultured, primed with IL-1α, and treated with cholesterol crystals under different concentrations. Inflammatory cytokines (mature-IL-1β, IL-6, and IL-8) in supernatant and inflammatory cytokines (pro-IL-1β, IL-18) in cell lysate were detected by western blot. The NF-κB pathway inhibitor BAY 11-7082 was used to determine the pathway of cytokine expression.

Purpose

To investigate the expression of inflammatory cytokines in ARPE-19 cells after stimulation with cholesterol crystals.

Results

Cholesterol crystals did not induce the nucleotide-binding domain leucine-rich repeat containing family, pyrin domain containing 3 (NLRP3) inflammasome, but did increase pro-IL-1β expression in ARPE-19 cells. Cholesterol crystals increased pro-IL-1β expression by activating the NF-κB pathway. Cholesterol crystal activation of the NF-κB pathway also leads to increased IL-6 and IL-8 expression.