Conclusion
Our results suggest that hypoxic PM was significantly associated with the positive regulation of Ca(2+) homeostasis in infarcted myocardium.
Methods
We isolated rat bone marrow-derived mesenchymal stem cells (MSCs), and prepared paracrine media (PM) from MSCs under hypoxic or normoxic conditions (hypoxic PM and normoxic PM). We induced rat myocardial infarction by left anterior descending ligation for 1 hour, and treated PM into the border region of infarcted myocardium (n=6/group) to identify the alteration in calcium-regulated proteins. We isolated and stained the heart tissue with specific calcium-related antibodies after 11 days.
Results
The hypoxic PM treatment increased Ca(2+)-related proteins such as L-type Ca(2+) channel, sarcoplasmic reticulum Ca(2+) ATPase, Na(+)/K(+) ATPase, and calmodulin, whereas the normoxic PM treatment increased those proteins only slightly. The sodium-calcium exchanger was significantly reduced by hypoxic PM treatment, compared to moderate suppression by the normoxic PM treatment.