Abstract
The complete mitochondrial genome of Cynoglossus joyneri was determined. The length of the C. joyneri complete mitochondrial DNA sequence is 16,428 bp. The tRNA Gln gene is translocated from the light to the heavy strand (Q inversion), which is accompanied by shuffling of the tRNA Ile gene and long-range translocation of the putative control region downstream to a site between ND1 and the tRNA Gln gene, which is also seen in the closely related Cynoglossus semilaevis. C. joyneri has a closer relationship to Cynoglossus lineolatus and C. semilaevis, but had a distant relationship to Phoxinus percnurus and Sternula albifrons.