Abstract
In this work, we reported the complete mitochondrial DNA sequence of the hybrid of Leiocassis longirostris (♂)×Pelteobagrus fulvidraco (♀), which was obtained by artificial hybridization. The total length of the mitochondrial genome is 16,527 bp, with the base composition of 30.86% for A, 25.56% for T, 28.20% for C, and 15.38% for G. It contains two ribosomal RNA genes, 13 protein-coding genes, 22 transfer RNA genes, and a major non-coding control region (D-loop region). The arrangement of these genes is the same as that found in the teleosts. All the protein initiation codons are ATG, except for COX1 that begins with GTG. The complete mitogenome of the hybrid provides an important data set for the study in mitochondrial inheritance mechanism. Both the termination-associated sequence and critical central conserved sequences (CSB-D, CSB-E, and CSB-F) were also detected.