Abstract
Chaetodontidae species feeding observations showed that they mostly fed on different coral species. Among them, Chaetodon speculum (Cuvier, 1831) is one of most important genera of Chaetodontidae, C. baronessa and C. bennetti appeared to ingest annelid worms during the course of coral feeding, whereas gut contents of C. punctatofasciatus and C. speculum were dominated by crustaceans. However, the systemically classification and taxonomic studies have so far been limited. In this study, we report the complete mitochondrial genome sequence of C. speculum. The mitogenome has 16,537 base pairs (54.4% A + T content) and made up of total of 37 genes (13 protein-coding, 22 transfer RNAs and 2 ribosomal RNAs), and a putative control region. This study will provide useful genetic information for future phylogenetic and taxonomic classification of Chaetodontidae.