Abstract
Toxins from toxin-antitoxin (TA) systems, widely distributed genetic elements in prokaryotic genomes, are traditionally viewed as growth inhibitors. This study challenges this view by uncovering a growth-promoting function for MazF, the toxin component of the Escherichia coli MazF/MazE TA system. MazF is a sequence-specific endoribonuclease that cleaves single-stranded RNA, including ribosomal RNA (rRNA) precursors. While ectopic overexpression of MazF causes growth arrest, we show here that, when expressed at its native expression levels, it alleviates both co-directional and head-on transcription-replication conflicts at highly transcribed rRNA operons. By resolving these conflicts, MazF mitigates DNA damage, reduces cell mortality, and ultimately facilitates growth resumption. This function is particularly critical during the exit from stationary phase, when the rRNA copy operon number is low or when ribosome integrity is compromised. We further demonstrate that MazF is active, indicating its release from MazE antitoxin control, during regrowth from stationary phase, heat shock at 50°C, and colistin treatment. Together, these findings reveal an unrecognized role of TA systems in promoting cellular resilience, maintaining genome stability, and enabling recovery from stress, thereby providing new insights into their complex biological functions.