Abstract
Genetic linkage maps are an essential tool in population genetics and plant breeding research, yet user-friendly online tools for constructing and visualizing them remain scarce. MSTmap Online addresses this gap by providing a modern, accessible platform for generating high-quality genetic linkage maps from genotypic data. The web server quickly computes linkage groups using the MSTmap algorithm and generates detailed output files, including publication-ready PDF visualizations of linkage groups. The server supports bookmarking and asynchronous processing, allowing users to revisit their results at a later time. A companion Python library for MSTmap Online enables seamless integration into custom analysis pipelines. MSTmap Online is free and open to all users with no login requirement at https://mstmap.org. The companion Python library is available at https://pypi.org/project/mstmap/.