Abstract
Currently, there is little scientific data to support the importance of selecting non-Saccharomyces yeasts from different wineries in the Protected Designation of Origin (PDO) in Andalusia, southern Spain, and how this group of yeasts can affect the sensory properties of wine. Therefore, this research aimed to study some specific microbiological properties and the metabolites they could produce in order to evaluate the oenological potential of two non-Saccharomyces yeast strains isolated from a region of Andalusia (Córdoba, Spain), Hanseniaspora uvarum TJ-27 and Lachancea thermotolerans T-9, isolated from musts with high sugar content. Of 80 yeast isolates selected, these two strains were chosen for their notable β-glucosidase activity (observed in up to 40% of isolates), cellulase activity (present in 24%), and killer phenotype (found in 40%). In this study, strains that displayed characteristics associated with aroma release were selected. Fermentation assays using a high-sugar synthetic medium revealed that neither H. uvarum TJ-27 nor L. thermotolerans T-9 was able to complete alcoholic fermentation independently, achieving ethanol yields of only 5-6% v/v, indicating the need for subsequent fermentation by Saccharomyces cerevisiae. The originality of this study provides insight into the metabolites contributed by these strains to the wines produced. The best results were obtained when both strains were inoculated together. Furthermore, volatilome analysis showed elevated levels of key compounds such as isoamyl alcohols and 2,3-butanediol. These findings highlight the practical potential of using selected non-Saccharomyces strains from Andalusia to improve fermentation results and wine quality. The novelty of this study lies mainly in confirmation within region-specific isolates.