Abstract
Pulmonary arterial hypertension (PAH) is a severe cardiopulmonary disease characterized by elevated pulmonary artery pressure and cardiac dysfunction, often leading to heart failure and even death. Despite the complexity of its mechanisms, the molecular basis of PAH remains unclear. tRNA-derived small RNAs (tsRNAs) are noncoding RNAs that play regulatory roles in various diseases. We collected plasma samples from PAH patients and healthy controls for small RNA microarray, and we established a monocrotaline-induced PAH rat model to collect right ventricular and lung tissues for tsRNA sequencing. Our analysis revealed 2,716 unique tsRNAs in human plasma and 4,733 in rat tissues, with a 7.84% overlap. Additionally, 204 tsRNAs were highly conserved across plasma, lung tissue, and right ventricle samples. The reproducibility of the expression profiles was confirmed through Pearson correlation and principal component analysis. KEGG pathway enrichment analysis indicated that tsRNAs are involved in key pathways, such as the MAPK and cancer signalling pathways. These datasets offer valuable insights for researching the epigenetic mechanisms underlying PAH and potential therapeutic targets.