Abstract
In this study, we assembled and annotated the mitochondrial genome (mitogenome) of B. oleracea L. var. italica Plenck. This mitogenome was found to span 219 964 bp, with a GC content of 45.25%. It comprised 61 genes, including 35 protein-coding, 23 transfer RNA, and three ribosomal RNA genes. Among these, only 11 genes contained introns. Codon preference analysis revealed a bias toward codons ending in A/U bases. A phylogenetic analysis demonstrated a close relationship between B. oleracea L. var. italica Plenck, B. oleracea L. botrytis, and B. oleracea var. capitata. This reference mitogenome provides a basis for research on genetic conservation, phylogenetic relationships, and molecular breeding strategies among members of the Brassica genus.