Abstract
The objective of this study was to evaluate the impact of blending, tuning, and scaling adjustments in ssGBLUP on the accuracy of genomic estimated breeding values (GEBVs) for South African Holstein cattle. The edited dataset included pedigree information for 541,325 animals, 696,413 phenotypic records (milk, protein, and fat yields), and genotypes for 1221 Holstein cattle. The accuracy of GEBVs was evaluated based on different parameter settings for blending (β = 0.05, 0.10, 0.20, 0.30, and 0.40), tuning (τ), and scaling (τ and ω), ranging from 0.60 to 1.00. The results show that ssGBLUP outperformed the traditional pedigree-based approach (ABLUP), with realized accuracies increasing from 0.01 to 0.23 for milk yield, 0.03 to 0.29 for protein yield, and 0.03 to 0.30 for fat yield. Blending with β = 0.30-0.40 slightly increased the accuracy, while tuning adjustments showed limited influence on the prediction results. Scaling factors had a significant influence on accuracy, with ω = 0.60 yielding the highest values (0.26 for milk, 0.32 for protein, and 0.34 for fat). The results of this study show the importance of optimizing the integration of pedigree and genomic information in ssGBLUP to improve the accuracy of genomic predictions, ultimately enhancing selection decisions and genetic progress in South African Holstein cattle.