Whole-Genome Draft Assemblies of Difficult-to-Classify Escherichia coli O157 and Non-O157 Isolates from Feces of Canadian Feedlot Cattle

从加拿大饲养场牛粪便中分离的难分类大肠杆菌 O157 和非 O157 菌株的全基因组草图

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作者:Vinicius Silva Castro, Eduardo Eustáquio de Souza Figueiredo, Tim McAllister, Robin King, Tim Reuter, Rodrigo Ortega Polo, Carlos Adam Conte Jr, Kim Stanford

Abstract

Forty-eight Escherichia coli strains were chosen due to variable detection of stx or serogroup by PCR. Although all strains were initially determined to be Shiga toxin-producing Escherichia coli (STEC), their genomes revealed 11 isolates carrying stx 1a, stx 1b, stx 2a, and/or stx 2b Assembled genome sizes varied between 4,667,418 and 5,556,121 bp, with N 50 values between 79,648 and 294,166 bp and G+C contents between 50.3% and 51.4%.

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