Abstract
In this study, we conducted a comprehensive sequencing and characterization of the chloroplast genome of the Asian endemic species Calystegia pubescens 'Anestia'. The complete genome is 152,079 bp long, with a GC content of 37.37%. It consists of a large single-copy region (87,891 bp), a small single-copy region (19,888 bp), and a pair of inverted repeats regions (22,149 bp). A total of 126 genes were identified, including 81 protein-coding genes, 37 tRNA genes, and eight rRNA genes. Phylogenetic analysis revealed a close relationship between C. pubescens 'Anestia' and C. hederacea. These results provide valuable genetic insights for species identification and phylogenetic studies within the genus Calystegia.