Abstract
Pittosporum illicioides Makino, 1900, a member of the genus Pittosporum, is a traditional medicinal plant extensively used in the Yao ethnic regions of southern China. The complete chloroplast genome of P. illicioides was sequenced and assembled for the first time, with a length of 153,610 bp, comprising a large single-copy (LSC) region (84,915 bp), a small single-copy (SSC) region (18,727 bp), and a pair of inverted repeat (IRa and IRb) regions (24,984 bp each) separated by the LSC and SSC regions. A total of 128 genes were annotated, including 83 protein-coding genes, 37 transfer RNA (tRNA) genes, and 8 ribosomal RNA (rRNA) genes. Among these, ten genes contained a single intron, while two genes (clpP and ycf3) possessed two introns. Phylogenetic analysis revealed that P. illicioides is most closely related to Pittosporum tobira. This plastome enhances genomic resources for Pittosporum and serves as a valuable reference for plant identification.