Estimation of Trace Elements Namely Lead, Copper, Selenium, and Cadmium by Acid Biopsy Technique in Sound and Carious Permanent Teeth Using Inductively Coupled Plasma-optical Emission Spectrometry: An In Vivo Study

利用电感耦合等离子体发射光谱法,通过酸蚀活检技术测定健康和龋齿恒牙中微量元素(铅、铜、硒和镉)的含量:一项体内研究

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Abstract

BACKGROUND: Presence of cariogenic trace elements alters crystal lattice and size of crystal, thereby increases the susceptibility of tooth to dental caries. So, the aim of the study is to estimate cariogenic trace elements such as lead, copper, selenium, and cadmium in sound and carious permanent teeth using acid etch biopsy technique and also to correlate the presence of trace elements and its cariogenicity of an individual. MATERIALS AND METHODS: A total of 805 children were screened, and 60 children were selected in the age-group of 12-14 years living in same locality and depending on caries status, 30 children in group I (caries-free group) and 30 children in group II [carious group with decayed, missing, and filled teeth (DMFT) score 2-3]. The 3 µL of 0.5 M perchloric acid was taken in Eppendorf pipette and applied over the isolated area for about 3 seconds and aspirated. RESULTS: Unpaired t-test was used to compare between groups, and there was statistical difference in trace elements between carious group and caries-free group in both gender. CONCLUSION: The trace elements value was found to be more in carious group compared to caries-free group. HOW TO CITE THIS ARTICLE: Kumar MS, Krishnan R, John JB, et al. Estimation of Trace Elements Namely Lead, Copper, Selenium, and Cadmium by Acid Biopsy Technique in Sound and Carious Permanent Teeth Using Inductively Coupled Plasma-optical Emission Spectrometry: An In Vivo Study. Int J Clin Pediatr Dent 2025;18(2):141-145.

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