Abstract
Glyptotendipes pallens (Meigen, 1804) serves as a significant bioindicator for monitoring freshwater ecosystems. Despite the performance of COI barcode analyses on G. pallens, the mitogenome of this taxon remains unassembled and unanalyzed. In this study, we provide one newly sequenced complete mitogenome of G. pallens for the first time. The mitogenome is 16,063 bp long with high A + T content, and consists of 13 protein-coding genes, 22 tRNA genes, two rRNA genes, and a noncoding control region. The phylogenetic analysis strongly supports G. pallens and G. tokunagai as sister species.