Abstract
Mosquito-borne pathogens are a global health burden. The realization of a scalable serum-free mosquito cell suspension cultivation method would enable the production of novel biotechnological products, including vaccines, recombinant proteins, and (modified) viruses for biological control. This study reports the adaptation of two Aedes-derived cell lines, C6/36 and U4.4, to serum-free suspension conditions using the chemically defined EX-CELL medium. Both cell lines achieved high cell concentrations (> 1.0 × 10(7) cells/mL) in shake flasks with minimal adaptation. The C6/36 suspension cultures were subsequently transferred to a 500 mL stirred tank bioreactor (STR), which likewise achieved high cell concentrations (1.3 × 10(7) cells/mL) and a doubling time of 31–36 h. As a proof of concept for the production of a virus in this system, C6/36 cells were cultivated in a STR and infected with the mosquito-borne flavivirus Usutu virus (USUV). These cultures produced high virus titers (> 3.6 × 10(8) TCID(50)/mL), with similar growth kinetics compared to adherent and shake flask cultures. This work is the first to show C6/36 mosquito cell cultivation in a bioreactor and demonstrates the potential of this serum-free suspension culture system for the efficient production of mosquito-infecting viruses. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1038/s41598-025-33792-z.